Structure of PAP-IgM FcK fusion protein with J-chain expressed in transgenic plant

Detection of weak receptor–ligand interactions using IgM and J-chain-based fusion proteins

Materials and methods IgM-based fusion proteins were generated by molecular cloning of the IgM backbone DNA sequence into the BTN2A2-IgG or PD-L1-IgG-encoding SigpIg+ vectors upstream of the IgG1 Fc part of the fusion protein. The secretory tailpiece of the human IgM heavy chain was also encoded in the human IgM heavy chain DNA sequence inserted (Mitoma J. et al. Nat Immunol .2007.8: 409-418). ...

Expression of a Human Prostatic Acid Phosphatase (PAP)-IgM Fc Fusion Protein in Plants Using In vitro Tissue Subculture

In this study, prostatic acid phosphatase (PAP), which is overexpressed in human prostate cancer cells, was cloned to be fused to the IgM constant fragment (Fc) for enhancing immunogenicity and expressed in transgenic tobacco plants. Then, the transgenic plants were propagated by in vitro tissue subculture. Gene insertion and expression of the recombinant PAP-IgM Fc fusion protein were confirme...

Chain-Deficient IgM Structural and Functional Analysis of J

some arrangement of inter-␮ bonds directs incorporation of J chain. IgM-S337 hexamer also dissociated to ␮2␬2 and ␮4␬4, but also yielded several species migrating much more slowly in SDS-PAGE than wild-type ␮12␬12. To account for these forms, we propose that each ␮-chain can interact with three other ␮-chains and that some hexameric molecules contain two catenated ␮6␬6 circles. J chain is a 15-...

Gene cloning and construction of prokaryotic and plant expression vectors of RICIN-A-Chain/PAP-S1 fusion protein and its inhibition of protein synthesis

A plant signal peptide-hepatitis B surface antigen fusion protein with enhanced stability and immunogenicity expressed in plant cells.

The use of transgenic plants to express orally immunogenic protein antigens is an emerging strategy for vaccine biomanufacturing and delivery. This concept has particular suitability for developing countries. One factor that has limited the development of this technology is the relatively modest levels of accumulation of some antigenic proteins in plant tissues. We used fusion protein design to...